"Ten kubek to prezent od mojej siostry."
Translation:This mug is a gift from my sister.
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The "od" definitely implies that my sister gave it to me, so it came "from" her.
"Present of my sister" would be the equivalent of "my sister's present", that is, somebody gave it to my sister at some point in the past, and it still belongs to her. That would be simple genitive with no preposition.