"The sisters are playing with the pens in the kitchen."
Translation:Die Schwestern spielen mit den Stiften in der Küche.
Yes, you could, but doing so would emphasise the "in der Küche" part of the sentence. Standard German word order is generally time - manner - place ("Küche" is the place so it would usually be last). When you change this order, you are emphasising the part of the sentence you decide to put first.