"Kyslík, jak ho známe, je plyn."
Translation:Oxygen as we know it is a gas.
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Unfortunately, being a native speaker is not per se a good prerequisite for any authority about placing commas. Not in Czech and not in English either. These are the things that must be learned - at school or somewhere else.
A brief search in Google Books or the example entry in https://www.merriam-webster.com/dictionary/as%20we%20know%20it does not support your statement.