"Kdo má žízeň?"

Translation: Who is thirsty?

[Andrew785114]

"Je" instead of "má", is that acceptable?

[VladaFu]

No, unless you use an adjective as well. Kdo je žíznivý? is theoretically possible, but less natural.

[pma4w89A]

You are thinking from English possibly backward into Czech. In English we say Who is thirsty, not Who has thirst, but Czech often uses have/ma' but ...
It is perfectly normal English to say have hunger this way ... Who said you have hunger pains? Me! You have hunger pains? Yes. But never I have hunger. While it would be understood, it's never used with "hunger" alone. But she/he/they had hunger pains they waited so long in line would be very normal in English. And there you see the connection to the Czech I have hunger, I have thirst. Hope that helps.