Translation:Do you speak it
Punctuation in Klingon does not have strict rules and is used more for the convenience of the reader. English can use a trick of placing the object at the front for emphasis and then referring back to it, as in, "English, do you speak it?". But since the object is already at the front in Klingon, it doesn't have as strong an impact. In speaking, you can imitate it a little, by giving a longer pause, but I'm not sure a comma conveys that well. I might suggest an ellipsis instead.
DIvI' Hol... Dajatlh'a'?
But if I remember correctly, in the movie that famously used the line, there's actually an epithet in between, which might work even better:
DIvI' Hol, petaQ, Dajatlh'a'?